Module 7 EN Welcome to your Module 7 EN The par-SeqFISH is a genome imaging approach that reveals the gene expression in a spatial context with a high resolution. FALSE TRUE None The single-cell transcriptomics with combinatorial barcoding approaches include the following methods: Probe-based bacterial single-cell DNA sequencing Seq-Well/Seq-Well3 Poly(A)-independent single-cell DNA- sequencing Microbial split-pool excision transcriptomics None The microbial consortia in environmental habitats comprise a complex and dynamic system and bear all the difficulties in their transcriptomics data generation and interpretation. FALSE TRUE None The principal protocol for the analysis of partial environmental transcriptomes comprises the following workflow: RNA extraction and multiplication, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and multiplication, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation None Transcripts annotation means assembly of constructs using either mapping against reference genomes or de novo assembly FALSE TRUE None Environmental transcriptomics retrieves transcriptomes (mRNA) of microbial assemblages from the environment to understand their interactions and the interactions with the environment. FALSE TRUE None The high-throughput methodologies, based on sequencing of single-cell RNA and supporting the single-cell resolution capability of the sequencing process include: HITdb, HOMD, FreshTrain MATQ-seq, par-SeqFISH, PETRI-seq HOMD, PETRI-seq, par-SeqFISH HITdb, HOMD, MATQ-seq None MATQ-seq techniques isolates single cells into separate wells of single-well plates and performs individual indexing reactions to generate sequencing libraries. TRUE FALSE None PETRI-seq is a technique that allows prokaryotic expression profiling by tagging RNA in situ followed by sequencing. TRUE FALSE None Seq-Well method explores droplets-based cell capture and barcoding. TRUE FALSE None Time's up Submit a Comment Cancel replyYou must be logged in to post a comment.
