Module 7 EN Welcome to your Module 7 EN The principal protocol for the analysis of partial environmental transcriptomes comprises the following workflow: RNA extraction and multiplication, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and multiplication, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation None The par-SeqFISH is a genome imaging approach that reveals the gene expression in a spatial context with a high resolution. FALSE TRUE None Some of the protocols that facilitate the analyses of partial environmental transcriptomes include: Meta-transcriptomics, spatial transcriptomics, and sorted transcriptomics Meta-transcriptomics, spatial transcriptomics, and fixed transcriptomics Meta-transcriptomics, temporal transcriptomics, and sorted transcriptomics Meta-transcriptomics, temporal transcriptomics, and fixed transcriptomics None The high-throughput methodologies, based on sequencing of single-cell RNA and supporting the single-cell resolution capability of the sequencing process include: HOMD, PETRI-seq, par-SeqFISH HITdb, HOMD, FreshTrain MATQ-seq, par-SeqFISH, PETRI-seq HITdb, HOMD, MATQ-seq None The microbial consortia in environmental habitats comprise a complex and dynamic system and bear all the difficulties in their transcriptomics data generation and interpretation. FALSE TRUE None Environmental proteomics possesses potential for generating innovative hypotheses about microbial processes. TRUE FALSE None The single-cell transcriptomics with combinatorial barcoding approaches include the following methods: Probe-based bacterial single-cell DNA sequencing Poly(A)-independent single-cell DNA- sequencing Microbial split-pool excision transcriptomics Seq-Well/Seq-Well3 None MATQ-seq techniques isolates single cells into separate wells of single-well plates and performs individual indexing reactions to generate sequencing libraries. TRUE FALSE None Seq-Well method explores droplets-based cell capture and barcoding. FALSE TRUE None Transcriptomics deals with RNA transcripts: Those parts of the genome that are transcribed in a particular cell, tissue, or organism at certain conditions Those parts of the genome that are translated in a particular cell, tissue, or organism at certain conditions Those parts of the genome that are always transcribed in a particular cell, tissue, or organism Those parts of the genome that are always translated in a particular cell, tissue, or organism None Time's up Submit a Comment Cancel replyYou must be logged in to post a comment.
