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2023-1-BG01-KA220-HED-000155777 – DigiOmica

Welcome to your Module 7 EN

Seq-Well method explores droplets-based cell capture and barcoding.

Transcriptomics deals with RNA transcripts:

MATQ-seq techniques isolates single cells into separate wells of single-well plates and performs individual indexing reactions to generate sequencing libraries.

The single-cell transcriptomics with combinatorial barcoding approaches include the following methods:

The ProBac-seq protocol exploits libraries of RNA probes and microfluidic commercial platforms to perform single-cell RNA sequencing.

The high-throughput methodologies, based on sequencing of single-cell RNA and supporting the single-cell resolution capability of the sequencing process include:

PETRI-seq is a technique that allows prokaryotic expression profiling by tagging RNA in situ followed by sequencing.

The par-SeqFISH is a genome imaging approach that reveals the gene expression in a spatial context with a high resolution.

Environmental proteomics possesses potential for generating innovative hypotheses about microbial processes.

Prokaryotic transcripts lack a polyadenylation mechanism and the DNA isolation is not easy.