Module 7 EN Welcome to your Module 7 EN The principal protocol for the analysis of partial environmental transcriptomes comprises the following workflow: RNA extraction and enrichment, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and multiplication, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and multiplication, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation None The single-cell transcriptomics with combinatorial barcoding approaches include the following methods: Microbial split-pool excision transcriptomics Probe-based bacterial single-cell DNA sequencing Poly(A)-independent single-cell DNA- sequencing Seq-Well/Seq-Well3 None PETRI-seq is a technique that allows prokaryotic expression profiling by tagging RNA in situ followed by sequencing. FALSE TRUE None Transcripts annotation means assembly of constructs using either mapping against reference genomes or de novo assembly FALSE TRUE None The ProBac-seq protocol exploits libraries of RNA probes and microfluidic commercial platforms to perform single-cell RNA sequencing. TRUE FALSE None MATQ-seq techniques isolates single cells into separate wells of single-well plates and performs individual indexing reactions to generate sequencing libraries. FALSE TRUE None Seq-Well method explores droplets-based cell capture and barcoding. TRUE FALSE None The high-throughput methodologies, based on sequencing of single-cell RNA and supporting the single-cell resolution capability of the sequencing process include: MATQ-seq, par-SeqFISH, PETRI-seq HITdb, HOMD, FreshTrain HITdb, HOMD, MATQ-seq HOMD, PETRI-seq, par-SeqFISH None Environmental proteomics possesses potential for generating innovative hypotheses about microbial processes. TRUE FALSE None Some of the protocols that facilitate the analyses of partial environmental transcriptomes include: Meta-transcriptomics, spatial transcriptomics, and fixed transcriptomics Meta-transcriptomics, spatial transcriptomics, and sorted transcriptomics Meta-transcriptomics, temporal transcriptomics, and fixed transcriptomics Meta-transcriptomics, temporal transcriptomics, and sorted transcriptomics None Time's up Submit a Comment Cancel replyYou must be logged in to post a comment.
