Module 7 EN Welcome to your Module 7 EN The ProBac-seq protocol exploits libraries of RNA probes and microfluidic commercial platforms to perform single-cell RNA sequencing. TRUE FALSE None The principal protocol for the analysis of partial environmental transcriptomes comprises the following workflow: RNA extraction and multiplication, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cRNA and amplification by PCR, sequencing of the cRNA clones and transcripts annotation RNA extraction and enrichment, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation RNA extraction and multiplication, synthesis of cDNA and amplification by PCR, sequencing of the cDNA clones and transcripts annotation None Environmental proteomics possesses potential for generating innovative hypotheses about microbial processes. TRUE FALSE None Prokaryotic transcripts lack a polyadenylation mechanism and the DNA isolation is not easy. FALSE TRUE None The high-throughput methodologies, based on sequencing of single-cell RNA and supporting the single-cell resolution capability of the sequencing process include: HITdb, HOMD, FreshTrain HOMD, PETRI-seq, par-SeqFISH MATQ-seq, par-SeqFISH, PETRI-seq HITdb, HOMD, MATQ-seq None PETRI-seq is a technique that allows prokaryotic expression profiling by tagging RNA in situ followed by sequencing. TRUE FALSE None The microbial consortia in environmental habitats comprise a complex and dynamic system and bear all the difficulties in their transcriptomics data generation and interpretation. TRUE FALSE None Transcripts annotation means assembly of constructs using either mapping against reference genomes or de novo assembly TRUE FALSE None Transcriptomics deals with RNA transcripts: Those parts of the genome that are always transcribed in a particular cell, tissue, or organism Those parts of the genome that are transcribed in a particular cell, tissue, or organism at certain conditions Those parts of the genome that are translated in a particular cell, tissue, or organism at certain conditions Those parts of the genome that are always translated in a particular cell, tissue, or organism None Seq-Well method explores droplets-based cell capture and barcoding. FALSE TRUE None Time's up Submit a Comment Cancel replyYou must be logged in to post a comment.
