Welcome to your Module 1 EN
Once extracted from a sample, the eDNA is not stable if preserved by freezing.
eDNA is used as a tool for the detection of targeted species and assessment of biodiversity in ecosystems.
The discovery and study of macroorganisms’ eDNA is essential for biodiversity conservation purposes.
eDNA metabarcoding comprises taking environmental samples, extracting RNA, amplifying it through PCR with general or universal primers, and sequencing the amplification product through NGS to generate a huge amount of raw sequencing data.
Environmental DNA (eDNA) is:
The different branches of genomics can be classified as:
The achievements of the eDNA approach in ancient ecosystems contribute to describing past biodiversity and shed light on macroorganisms' evolution history.
eDNA protocols comprise the following CONSEQUENT procedures:
Genomics is a bioscience that analyses the interactions of genes as biological information objects at species, population, and ecosystem levels.
Which of the following IS NOT a protocol’s error-prone step:
The microarray genetics includes genetic approaches, complementary to each other: forward and reverse.
The relatively fast dispersion and low concentration of aquatic eDNA make the traditional methods for microbial species assessment in aquatic samples simple.
Next-generation sequencing (NGS) is a massively parallel sequencing technology that offers high speed and scalability of the sequencing process, together with deep sequencing of target DNA regions and a high results preciseness.
Environmental genomics integrates environmental data and metadata accumulated at various levels of organisms’ organization
