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The catastrophic 2010 Deepwater Horizon oil spill in the Gulf of Mexico led to the activation of natural microbial communities with oil-degrading capacity. To identify the key bacteria responsible for natural bioremediation, scientists used:
eDNA metabarcoding comprises taking environmental samples, extracting RNA, amplifying it through PCR with general or universal primers, and sequencing the amplification product through NGS to generate a huge amount of raw sequencing data.