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2023-1-BG01-KA220-HED-000155777 – DigiOmica

Welcome to your CLP 6 EN Exam

Omics technologies allow us to understand the effects of environmental pollutants at the genetic and molecular level.

The ProBac-seq protocol exploits libraries of RNA probes and microfluidic commercial platforms to perform single-cell RNA sequencing.

eDNA protocols comprise the following CONSEQUENT procedures:

Genomic approaches can examine epigenetic changes such as DNA methylation caused by toxic substances.

eDNA metabarcoding comprises taking environmental samples, extracting RNA, amplifying it through PCR with general or universal primers, and sequencing the amplification product through NGS to generate a huge amount of raw sequencing data.

Transcriptomics deals with RNA transcripts:

Omics technologies can enable the assessment of toxic effects both in individual organisms and at the ecosystem level.

Environmental proteomics catalogues the proteins of ecosystem parts, even lacking the knowledge about the organisms producing those proteins.

From technical point of view, the metaproteomic approach provides great advantage compared to standard biochemical/molecular biology analyses.

One of the difficulties encountered in the application of omics technologies is the limited reference databases for aquatic species.